MCP-1/CCL2 Recombinant antibody

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  WB analysis of NR8383 using 83370-2-RR

  Untreated, LPS and Brefeldin A treated NR8383 cells were subjected to SDS PAGE followed by western blot with 83370-2-RR (Ccl2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83370-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83370-2-RR against Rat MCP-1 were performed. The affinity constant is 0.836 nM.

MCP-1/CCL2 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 83370-2-RR

Clone No.240341G3

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, ELISA

Synonyms

Ccl2, chemokine (C C motif) ligand 2, CCL-2, CCL 2, C-C motif chemokine 2

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Tested Applications

• Positive WB detected in: LPS and Brefeldin A treated NR8383 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83370-2-RR targets MCP-1/CCL2 in WB, ELISA applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: chemokine (C-C motif) ligand 2
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: NM_031530
• Gene Symbol: MCP-1/CCL2
• Gene ID (NCBI): 24770
• RRID: AB_3671024
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P14844
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Monocyte chemoattractant protein-1 (MCP-1/CCL2) is one of the key chemokines that regulate migration and infiltration of monocytes/macrophages. Both CCL2 and its receptor CCR2 have been demonstrated to be induced and involved in various diseases. Migration of monocytes from the blood stream across the vascular endothelium is required for routine immunological surveillance of tissues, as well as in response to inflammation.