MCP-1/CCL2 Polyclonal antibody

• 

  WB analysis using 31520-1-AP

  Untreated, LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 31520-1-AP (Ccl2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

MCP-1/CCL2 Polyclonal Antibody for WB, ELISA

Cat No. 31520-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

Ccl2, Chemokine C C motif ligand 2, chemokine (C C motif) ligand 2, CCL-2, CCL 2

Formulation:  PBS and Azide

PBS and Azide

Conjugate:  Unconjugated

Unconjugated

Size/Concentration: 

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Freight/Packing: -

Quantity

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Tested Applications

• Positive WB detected in: LPS and Protein transport inhibitor treated RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31520-1-AP targets MCP-1/CCL2 in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: chemokine (C-C motif) ligand 2
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: NM_031530
• Gene Symbol: MCP-1/CCL2
• Gene ID (NCBI): 24770
• RRID: AB_3670017
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P14844
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Monocyte chemoattractant protein-1 (MCP-1/CCL2) is one of the key chemokines that regulate migration and infiltration of monocytes/macrophages. Both CCL2 and its receptor CCR2 have been demonstrated to be induced and involved in various diseases. Migration of monocytes from the blood stream across the vascular endothelium is required for routine immunological surveillance of tissues, as well as in response to inflammation.