Caveolin-1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Caveolin-1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

6C2B2

Cat no : 66067-1-PBS

Synonyms

CAV1

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Validation Data Gallery

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  WB analysis using 66067-1-Ig (same clone as 66067-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66067-1-Ig (same clone as 66067-1-PBS)

  WB result of Caveolin-1 antibody (66067-1-Ig; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caveolin-1 transfected HeLa cells. This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66067-1-Ig (same clone as 66067-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66067-1-Ig (same clone as 66067-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66067-1-Ig (same clone as 66067-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66067-1-Ig (same clone as 66067-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66067-1-Ig (same clone as 66067-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66067-1-Ig (same clone as 66067-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 66067-1-Ig (same clone as 66067-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Caveolin-1 antibody (66067-1-Ig, Clone: 6C2B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 66067-1-Ig (same clone as 66067-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Caveolin-1 antibody (66067-1-Ig, Clone: 6C2B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human skin cancer using 66067-1-Ig (same clone as 66067-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human skin cancer tissue using Caveolin-1 antibody (66067-1-Ig, Clone: 6C2B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human skin cancer using 66067-1-Ig (same clone as 66067-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human skin cancer tissue using Caveolin-1 antibody (66067-1-Ig, Clone: 6C2B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66067-1-PBS targets Caveolin-1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Caveolin-1 fusion protein Ag8049
• Full Name: caveolin 1, caveolae protein, 22kDa
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 20-25 kDa
• GenBank Accession Number: BC006432
• Gene Symbol: CAV1
• Gene ID (NCBI): 857
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor (PMID: 19641024). Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma (PMID: 19002186). However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer (PMID: 20558341). Recent study also show that CAV1 is involved in gastric Cancer (PMID: 25339030).