Cathepsin L Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Cathepsin L Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A12G8

Cat no : 66914-1-PBS

Synonyms

Cathepsin L, cathepsin L1, CATL, CTSL, CTSL1, FLJ31037, Major excreted protein, MEP

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Validation Data Gallery

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  WB analysis of A549 using 66914-1-Ig (same clone as 66914-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66914-1-Ig (Cathepsin L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66914-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 66914-1-Ig (same clone as 66914-1-PBS)

  WB result of Cathepsin L antibody (66914-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cathepsin L transfected A549 cells. This data was developed using the same antibody clone with 66914-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66914-1-Ig (same clone as 66914-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66914-1-Ig (Cathepsin L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66914-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NCI-H1299 using 66914-1-Ig (same clone as 66914-1-PBS)

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 66914-1-Ig (Cathepsin L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66914-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HUVEC using 66914-1-Ig (same clone as 66914-1-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 66914-1-Ig (Cathepsin L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66914-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66914-1-Ig (same clone as 66914-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66914-1-Ig (Cathepsin L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66914-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66914-1-Ig (same clone as 66914-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66914-1-Ig (Cathepsin L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66914-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66914-1-PBS targets Cathepsin L in WB, IHC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cathepsin L fusion protein Ag27951
• Full Name: cathepsin L1
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC012612
• Gene Symbol: CTSL1
• Gene ID (NCBI): 1514
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CTSL1(Cathepsin L1) is also named as CTSL and MEP, belongs to the peptidase C1 family. It is a lysosomal proteinase whose expression is also up-regulated in the skeletal muscle during starvation(PMID:20088826). It plays an intracellular role in normal intestinal epithelial polarization and initiation of neoplasia(PMID:17622569). CTSL1 also improves cardiac function and inhibits cardiac hypertrophy, inflammation, and fibrosis through blocking AKT/GSK3B signaling(PMID:19096818). The full length protein is 38 kDa with a signal peptide, two propeptide and a glycosylation site. It has been detected the 36 kDa, 39 kDa, 29 kDa and 21 kDa in rat gastrocnemius muscle. These forms of cathepsin L could either be attributed to differences in glycosylation or to partial processing of the proenzyme. (PMID:11696001)