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  • Featured Product
  • KD/KO Validated

Cathepsin L Monoclonal antibody

Cathepsin L Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A12G8

Cat no : 66914-1-Ig

Synonyms

CTSL1, CATL, cathepsinL1, cathepsin L1, Cathepsin L light chain

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using Cathepsin L Monoclonal antibody (66914-1-Ig)

    WB analysis of A549 using 66914-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 66914-1-Ig (Cathepsin L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using Cathepsin L Monoclonal antibody (66914-1-Ig)

    WB analysis of A549 using 66914-1-Ig

    WB result of Cathepsin L antibody (66914-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cathepsin L transfected A549 cells.

  • Western Blot (WB) analysis of HeLa cells using Cathepsin L Monoclonal antibody (66914-1-Ig)

    WB analysis of HeLa using 66914-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66914-1-Ig (Cathepsin L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NCI-H1299 cells using Cathepsin L Monoclonal antibody (66914-1-Ig)

    WB analysis of NCI-H1299 using 66914-1-Ig

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 66914-1-Ig (Cathepsin L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HUVEC cells using Cathepsin L Monoclonal antibody (66914-1-Ig)

    WB analysis of HUVEC using 66914-1-Ig

    HUVEC cells were subjected to SDS PAGE followed by western blot with 66914-1-Ig (Cathepsin L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using Cathepsin L Monoclonal antibody (66914-1-Ig)

    WB analysis of HepG2 using 66914-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66914-1-Ig (Cathepsin L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Cathepsin L Monoclonal antibody (66914-1-Ig)

    IHC staining of human prostate cancer using 66914-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66914-1-Ig (Cathepsin L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using Cathepsin L Monoclonal antibody (66914-1-Ig)

    IF Staining of HEK-293 using 66914-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using Cathepsin L antibody (66914-1-Ig, Clone: 1A12G8 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using Cathepsin L Monoclonal antibody (66914-1-Ig)

    IF Staining of HEK-293 using 66914-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using Cathepsin L antibody (66914-1-Ig, Clone: 1A12G8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

Tested Applications

Positive WB detected inA549 cells, NCI-H1299 cells, HUVEC cells, HepG2 cells, HeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66914-1-Ig targets Cathepsin L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Cathepsin L fusion protein Ag27951
Full Name cathepsin L1
Calculated Molecular Weight 38 kDa
Observed Molecular Weight43 kDa
GenBank Accession NumberBC012612
Gene Symbol Cathepsin L
Gene ID (NCBI) 1514
RRIDAB_2882241
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTSL1(Cathepsin L1) is also named as CTSL and MEP, belongs to the peptidase C1 family. It is a lysosomal proteinase whose expression is also up-regulated in the skeletal muscle during starvation(PMID:20088826). It plays an intracellular role in normal intestinal epithelial polarization and initiation of neoplasia(PMID:17622569). CTSL1 also improves cardiac function and inhibits cardiac hypertrophy, inflammation, and fibrosis through blocking AKT/GSK3B signaling(PMID:19096818). The full length protein is 38 kDa with a signal peptide, two propeptide and a glycosylation site. It has been detected the 36 kDa, 39 kDa, 29 kDa and 21 kDa in rat gastrocnemius muscle. These forms of cathepsin L could either be attributed to differences in glycosylation or to partial processing of the proenzyme. (PMID:11696001)

Protocols

Product Specific Protocols
WB protocol for Cathepsin L antibody 66914-1-IgDownload protocol
IHC protocol for Cathepsin L antibody 66914-1-IgDownload protocol
IF protocol for Cathepsin L antibody 66914-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Clin Sci (Lond)

Loss of FoxO1 activates an alternate mechanism of mitochondrial quality control for healthy adipose browning

Authors - Limin Shi
View Article
humanWB

Cancer Sci

Methyltransferase-like 3 promotes cervical cancer metastasis by enhancing cathepsin L mRNA stability in an N6-methyladenosine-dependent manner

Authors - Pingping Liu
  • KD Validated
View Article