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Cathepsin D Recombinant antibody, PBS Only

Cathepsin D Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240775G2

Cat no : 83743-5-PBS

Synonyms

CTSD, CPSD, CLN10, Cathepsin D light chain, Cathepsin D heavy chain

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Cathepsin D Recombinant antibody (83743-5-RR)

    IF Staining of HepG2 using 83743-5-RR (same clone as 83743-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cathepsin D antibody (83743-5-RR, Clone: 240775G2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83743-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83743-5-RR

    Affinity and Kinetic Characterization of 83743-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83743-5-RR against Human Cathepsin D were performed. The affinity constant is 0.821 nM.

Product Information

The immunogen of 83743-5-PBS is Cathepsin D Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name cathepsin D
Calculated Molecular Weight45kDa
GenBank Accession NumberNM_001909.5
Gene Symbol Cathepsin D
Gene ID (NCBI) 1509
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CTSD (Cathepsin D) also named CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 34-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease (PMID: 27114232, PMID: 30717773, PMID: 30051532).