Cathepsin D Polyclonal antibody, PBS Only

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  WB analysis using 55021-1-AP (same clone as 55021-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55021-1-AP (Cathepsin D antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55021-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 55021-1-AP (same clone as 55021-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55021-1-AP (Cathepsin D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55021-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 55021-1-AP (same clone as 55021-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55021-1-AP (Cathepsin D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55021-1-PBS in a different storage buffer formulation.

Cathepsin D Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 55021-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

55021-1-PBS targets Cathepsin D in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cathepsin D
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: NM_001909
• Gene Symbol: Cathepsin D
• Gene ID (NCBI): 1509
• RRID: AB_10863128
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P07339
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

CTSD (Cathepsin D) is also named as CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 34-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. (PMID: 27114232, PMID: 30717773, PMID: 30051532)