Caspase 8/P43/P18 Polyclonal antibody, PBS Only

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  WB analysis of Jurkat using 13423-1-AP (same clone as 13423-1-PBS)

  Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (Caspase 8/P43/P18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

• 

  WB analysis of UV treated Hela using 13423-1-AP (same clone as 13423-1-PBS)

  UV treated Hela cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (Caspase 8/P43/P18 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW264.7 using 13423-1-AP (same clone as 13423-1-PBS)

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (Caspase 8/P43/P18 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 13423-1-AP (same clone as 13423-1-PBS)

  IP result of anti-Caspase 8/P43/P18 (IP:13423-1-AP, 3ug; Detection:13423-1-AP 1:500) with HeLa cells lysate 2500ug. This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

• 

  IP experiment of Jurkat using 13423-1-AP (same clone as 13423-1-PBS)

  IP result of anti-Caspase 8/P43/P18 (IP:13423-1-AP, 4ug; Detection:13423-1-AP 1:500) with Jurkat cells lysate 2880 ug. This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 13423-1-AP (same clone as 13423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13423-1-AP (Caspase 8/P43/P18 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 13423-1-AP (same clone as 13423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13423-1-AP (Caspase 8/P43/P18 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 13423-1-AP (same clone as 13423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13423-1-AP (Caspase 8/P43/P18 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 13423-1-AP (same clone as 13423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 13423-1-AP (Caspase 8/P43/P18 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 13423-1-AP (same clone as 13423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 13423-1-AP (Caspase 8/P43/P18 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 13423-1-AP (same clone as 13423-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Caspase 8/P43/P18 antibody (13423-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Caspase 8/P43/P18 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13423-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CASP8, Caspase 8, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4

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Product Information

13423-1-PBS targets Caspase 8/P43/P18 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Caspase 8/P43/P18 fusion protein Ag4214
• Full Name: caspase 8, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 538 aa, 62 kDa
• Observed Molecular Weight: 53-57 kDa, 32-45 kDa
• GenBank Accession Number: BC028223
• Gene Symbol: Caspase 8
• Gene ID (NCBI): 841
• RRID: AB_2068463
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14790
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CASP8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. CASP8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816 ). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). CASP8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize the pro- and cleaved-caspase 8.