Cleaved Caspase 8/P18 Recombinant antibody

• 

  WB analysis using 85171-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85171-3-RR (Cleaved Caspase 8/P18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Cleaved Caspase 8/P18 {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 85171-3-RR

Clone No.242445E5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CASP8, Caspase 8, 242445E5, Apoptotic cysteine protease, Apoptotic protease Mch-5

---

Tested Applications

• Positive WB detected in: Staurosporine treated Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85171-3-RR targets Cleaved Caspase 8/P18 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: caspase 8, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 538 aa, 62 kDa
• Observed Molecular Weight: 55 kDa, 18 kDa
• GenBank Accession Number: BC028223
• Gene Symbol: Caspase 8
• Gene ID (NCBI): 841
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14790
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CASP8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. CASP8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816 ). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). This antibody recognize the cleaved caspase 8.