Cleaved Caspase 7/P20 Recombinant antibody

• 

  WB analysis using 85328-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85328-2-RR (Cleaved Caspase 7/P20 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Cleaved Caspase 7/P20 {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 85328-2-RR

Clone No.242407F6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CASP7, Caspase 7, 242407F6, Apoptotic protease Mch 3, Apoptotic protease Mch-3

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Tested Applications

• Positive WB detected in: Staurosporine treated Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85328-2-RR targets Cleaved Caspase 7/P20 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: caspase 7, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 303 aa, 34 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC015799
• Gene Symbol: Caspase 7
• Gene ID (NCBI): 840
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P55210
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspase 7(CASP7), like caspases 3 and 6, contains a short prodomain and, upon apoptotic induction, the 35 kDa proform is converted into a 32 kDa intermediate or preactive form which is further processed into two active subunits consisting of the p20 or large (18 kDa) subunit and the p10 or small (11 kDa) subunit and it is present in the brain, which is up-regulated and activated after traumatic injury(PMID:15953353). Caspase-7 is classified as a member of the subgroup of cysteine proteases most related to the Caenorhabditis elegans factor CED-3, which also includes caspase-3, -6, and -9(PMID:9426061). The protein is involved in the activation cascade of caspases responsible for apoptosis execution. This antibody recognize the cleaved caspase 7.