CoraLite® Plus 488-conjugated Caspase 4 Monoclonal antibody

Caspase 4 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D1E12

Cat no : CL488-67398

Synonyms

CASP 4, CASP4, Caspase 4, Caspase4, ICE(rel) II, ICE(rel)II, ICEREL II, ICH 2, ICH2, Mih1/TX, Protease ICH 2, Protease TX, TX

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Validation Data Gallery

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  IF Staining of HeLa using CL488-67398

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 Caspase 4 antibody (CL488-67398, Clone: 1D1E12 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67398 targets Caspase 4 in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Caspase 4 fusion protein Ag29488
• Full Name: caspase 4, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 377 aa, 43 kDa
• Observed Molecular Weight: 43-48 kDa
• GenBank Accession Number: BC017839
• Gene Symbol: Caspase 4
• Gene ID (NCBI): 837
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Caspase 4 (CASP4), is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.Caspase 4 is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. Overexpression of caspase 4 will induce cell apoptosis.