Filter: AllWBIHCIF-PIF/ICC

WB analysis of SGC-7901 using 67398-1-Ig (same clone as 67398-1-PBS)
SGC-7901 cells were subjected to SDS PAGE followed by western blot with 67398-1-Ig (Caspase 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.
WB analysis of HL-60 using 67398-1-Ig (same clone as 67398-1-PBS)
HL-60 cells were subjected to SDS PAGE followed by western blot with 67398-1-Ig (Caspase 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.
WB analysis of A549 using 67398-1-Ig (same clone as 67398-1-PBS)
A549 cells were subjected to SDS PAGE followed by western blot with 67398-1-Ig (Caspase 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 67398-1-Ig (same clone as 67398-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 67398-1-Ig (Caspase 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 67398-1-Ig (same clone as 67398-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 67398-1-Ig (Caspase 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 67398-1-Ig (same clone as 67398-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67398-1-Ig (Caspase 4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 67398-1-Ig (same clone as 67398-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67398-1-Ig (Caspase 4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.
IF Staining of human liver cancer using 67398-1-Ig (same clone as 67398-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Caspase 4 antibody (67398-1-Ig, Clone: 1D1E12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.
IF Staining of human liver cancer using 67398-1-Ig (same clone as 67398-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Caspase 4 antibody (67398-1-Ig, Clone: 1D1E12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 67398-1-Ig (same clone as 67398-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Caspase 4 antibody (67398-1-Ig, Clone: 1D1E12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.

Caspase 4 Monoclonal antibody, PBS Only

Caspase 4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67398-1-PBS

Clone No.1D1E12

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CASP4, Caspase4, Caspase 4/p20/p10, CASP-4, CASP 4

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67398-1-PBS targets Caspase 4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	Caspase 4 fusion protein Ag29488 Predict reactive species
Full Name	caspase 4, apoptosis-related cysteine peptidase
Calculated Molecular Weight	377 aa, 43 kDa
Observed Molecular Weight	43-48 kDa
GenBank Accession Number	BC017839
Gene Symbol	Caspase 4
Gene ID (NCBI)	837
RRID	AB_2882641
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P49662
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

Caspase 4 (CASP4), is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.Caspase 4 is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. Overexpression of caspase 4 will induce cell apoptosis.