CoraLite® Plus 488-conjugated Caspase 3/p17/p19 Polyclonal antibody

• Featured Product
• KD/KO Validated

Caspase 3/p17/p19 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-19677

Synonyms

CASP3, Caspase3, Caspase-3, Caspase 3, CASP-3

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Validation Data Gallery

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  IF Staining of NIH/3T3 using CL488-19677

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite® Plus 488 Caspase 3/p17/p19 antibody (CL488-19677) at dilution of 1:100.

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  IF Staining of HeLa using CL488-19677

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 Caspase 3/p17/p19 antibody (CL488-19677) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells, NIH/3T3 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-19677 targets Caspase 3/p17/p19 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: caspase 3, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32-35 kDa, 17 kDa, 19 kDa
• GenBank Accession Number: NM_004346
• Gene Symbol: Caspase 3
• Gene ID (NCBI): 836
• RRID: AB_2919166
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.