Caspase 10 Recombinant antibody

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  WB analysis using 85196-3-RR

  Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 85196-3-RR (Caspase 10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85196-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85196-3-RR (Caspase 10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  FC experiment of HepG2 using 85196-3-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Caspase 10 Recombinant Antibody (85196-3-RR, Clone:242353H6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Caspase 10 {ptg:Brand} Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 85196-3-RR

Clone No.242353H6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

CASP10, Caspase10, CASP-10, CASP 10, Apoptotic protease Mch-4

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Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells, A431 cells, K-562 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85196-3-RR targets Caspase 10 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: caspase 10, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC042844
• Gene Symbol: Caspase 10
• Gene ID (NCBI): 843
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q92851
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CASP10(Caspase-10) is also named as MCH4 and belongs to the peptidase C14A family. It is recruited to the CD99 and p55 tumor necrosis factor receptor signaling complexes and promoting CD95 (APO1/Fas) apoptosis. It may also play a critical role in PTK7-knockdown-induced apoptosis, downstream of mitochondria(PMID:21103379). This protein can be Cleavage by granzyme B and autocatalytic activity generate two active subunits. Defects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A)(PMID:16446975), familial non-Hodgkin lymphoma (NHL)(PMID:12010812) and gastric cancer (GASC)(PMID:11973654). The full length has a propeptide with 219 amino acid and 6 isoforms produced by alternative splicing.