Caspase 1 Recombinant antibody

• Featured Product
• KD/KO Validated

Caspase 1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

23

CloneNo.

1O18

Cat no : 81482-1-RR

Synonyms

CASP1, Caspase1, Caspase-1, CASP-1, CASP 1

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Validation Data Gallery

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  WB analysis using 81482-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81482-1-RR (Caspase 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 81482-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81482-1-RR against Human Caspase 1 were performed. The affinity constant is 305 nM.

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Tested Applications

• Positive WB detected in: Raji cells, THP-1 cells, U-937 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 23 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

81482-1-RR targets Caspase 1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Caspase 1 fusion protein Ag11143
• Full Name: caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
• Calculated Molecular Weight: 404 aa, 45 kDa
• Observed Molecular Weight: 30 kDa, 35 kDa, 43-45 kDa
• GenBank Accession Number: BC062327
• Gene Symbol: Caspase 1
• Gene ID (NCBI): 834
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products (PMID:21804020). It has 5 isoforms produced by alternative splicing. 81482-1-RR can recognize the splicing isoforms of caspase 1.

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); Scutellarin Rescued Mitochondrial Damage through Ameliorating Mitochondrial Glucose Oxidation via the Pdk-Pdc Axis; Authors - Ning Sheng
rat	WB,IF	Phytomedicine; Systemic pharmacology reveal the mechanism by which the Qiangjin Zhuanggu Qufeng mixture inhibits LPS-induced pyroptosis of rat nucleus pulposus cells; Authors - Dong Wang
mouse	WB	Int Immunopharmacol; Triggering receptor expressed on myeloid cells-1 aggravates obliterative bronchiolitis via enhancing the proinflammatory phenotype of macrophages; Authors - Junhao Wan
mouse	WB	Antioxidants (Basel); Bisdemethoxycurcumin Alleviates Dextran Sodium Sulfate-Induced Colitis via Inhibiting NLRP3 Inflammasome Activation and Modulating the Gut Microbiota in Mice; Authors - Jingfei Zhang
rat	WB	PLoS One; Forsythoside B alleviates cerebral ischemia-reperfusion injury via inhibiting NLRP3 inflammasome mediated by SIRT1 activation; Authors - Qiaoyu Li
mouse	WB	Biochem Biophys Res Commun; Uric acid-lowering effect of harpagoside and its protective effect against hyperuricemia-induced renal injury in mice; Authors - Qiong Fu