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Caspase 1 Recombinant antibody, PBS Only

Caspase 1 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1O18

Cat no : 81482-1-PBS

Synonyms

CASP1, Caspase1, Caspase-1, CASP-1, CASP 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Caspase 1 Recombinant antibody (81482-1-RR)

    WB analysis using 81482-1-RR (same clone as 81482-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81482-1-RR (Caspase 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81482-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81482-1-RR

    Affinity and Kinetic Characterization of 81482-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81482-1-RR against Human Caspase 1 were performed. The affinity constant is 305 nM.

Product Information

81482-1-PBS targets Caspase 1 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Caspase 1 fusion protein Ag11143
Full Name caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
Calculated Molecular Weight 404 aa, 45 kDa
Observed Molecular Weight30 kDa, 35 kDa, 43-45 kDa
GenBank Accession NumberBC062327
Gene Symbol Caspase 1
Gene ID (NCBI) 834
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products (PMID:21804020). It has 5 isoforms produced by alternative splicing. 81482-1-RR can recognize the splicing isoforms of caspase 1.