Cardiac Troponin T Monoclonal antibody, PBS Only (Capture)

Cardiac Troponin T Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

mouse, rat, pig, rabbit, chicken

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4E2D8

Cat no : 68300-1-PBS

Synonyms

TNNT2, cTnT, Troponin T, cardiac muscle, 4E2D8

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Validation Data Gallery

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  WB analysis of pig heart using 68300-1-Ig (same clone as 68300-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 68300-1-Ig (cTnT antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68300-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit heart using 68300-1-Ig (same clone as 68300-1-PBS)

  rabbit heart tissue were subjected to SDS PAGE followed by western blot with 68300-1-Ig (cTnT antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68300-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 68300-1-Ig (same clone as 68300-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 68300-1-Ig (cTnT antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68300-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 68300-1-Ig (same clone as 68300-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 68300-1-Ig (cTnT antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68300-1-PBS in a different storage buffer formulation.

• 

  WB analysis of chicken heart using 68300-1-Ig (same clone as 68300-1-PBS)

  chicken heart tissue were subjected to SDS PAGE followed by western blot with 68300-1-Ig (cTnT antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68300-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat heart using 68300-1-Ig (same clone as 68300-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 68300-1-Ig (Cardiac Troponin T antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68300-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 68300-1-Ig (same clone as 68300-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using cTnT antibody (68300-1-Ig, Clone: 4E2D8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68300-1-PBS in a different storage buffer formulation.

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  IF Staining of H9C2 using 68300-1-Ig (same clone as 68300-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using Cardiac Troponin T antibody (68300-1-Ig, Clone: 4E2D8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68300-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50338-1

  Cytometric bead array standard curve of MP50338-1, cTnT Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68300-1-PBS. Detection antibody: 68300-2-PBS. Standard:Ag7716. Range: 0.391-50 ng/mL.

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Product Information

68300-1-PBS targets Cardiac Troponin T as part of a matched antibody pair:

MP50338-1: 68300-1-PBS capture and 68300-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse, rat, pig, rabbit, chicken
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cardiac Troponin T fusion protein Ag7716
• Full Name: troponin T type 2 (cardiac)
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 34-40 kDa
• GenBank Accession Number: BC002653
• Gene Symbol: Cardiac Troponin T
• Gene ID (NCBI): 7139
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Cardiac Troponin T, also named as cTnT, TNNT2. This gene encodes the cardiac isoform of troponin T. The encoded protein is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Cardiac Troponin T can be detected as about 34-40 kDa.