Cardiac Troponin T Monoclonal antibody, PBS Only

Cardiac Troponin T Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

mouse, rat, rabbit, pig, chicken

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4E2D8

Cat no : 68300-1-PBS

Synonyms

Cardiac muscle troponin T, CMH2, CMPD2, cTnT, RCM3, TNNT2, TnTC, troponin T type 2 (cardiac), Troponin T, cardiac muscle

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Validation Data Gallery

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  WB analysis of pig heart using 68300-1-Ig (same clone as 68300-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 68300-1-Ig (cTnT antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68300-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit heart using 68300-1-Ig (same clone as 68300-1-PBS)

  rabbit heart tissue were subjected to SDS PAGE followed by western blot with 68300-1-Ig (cTnT antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68300-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 68300-1-Ig (same clone as 68300-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 68300-1-Ig (cTnT antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68300-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 68300-1-Ig (same clone as 68300-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 68300-1-Ig (cTnT antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68300-1-PBS in a different storage buffer formulation.

• 

  WB analysis of chicken heart using 68300-1-Ig (same clone as 68300-1-PBS)

  chicken heart tissue were subjected to SDS PAGE followed by western blot with 68300-1-Ig (cTnT antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68300-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat heart using 68300-1-Ig (same clone as 68300-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 68300-1-Ig (Cardiac Troponin T antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68300-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 68300-1-Ig (same clone as 68300-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using cTnT antibody (68300-1-Ig, Clone: 4E2D8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68300-1-PBS in a different storage buffer formulation.

• 

  IF Staining of H9C2 using 68300-1-Ig (same clone as 68300-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using Cardiac Troponin T antibody (68300-1-Ig, Clone: 4E2D8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68300-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68300-1-PBS targets Cardiac Troponin T in WB, IF, IHC, Indirect ELISA applications and shows reactivity with mouse, rat, rabbit, pig, chicken samples.

• Tested Reactivity: mouse, rat, rabbit, pig, chicken
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cardiac Troponin T fusion protein Ag7716
• Full Name: troponin T type 2 (cardiac)
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 34-40 kDa
• GenBank Accession Number: BC002653
• Gene Symbol: TNNT2
• Gene ID (NCBI): 7139
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Cardiac Troponin T, also named as cTnT, TNNT2. This gene encodes the cardiac isoform of troponin T. The encoded protein is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Cardiac Troponin T can be detected as about 34-40 kDa.