Cardiac Troponin T Monoclonal antibody

Cardiac Troponin T Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit, chicken

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

4E2D8

Cat no : 68300-1-Ig

Synonyms

TNNT2, cTnT, Troponin T, cardiac muscle, 4E2D8



Tested Applications

Positive WB detected inrat heart tissue, pig heart tissue, rabbit heart tissue, mouse heart tissue, chicken heart tissue
Positive IHC detected inrat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue
Positive IF/ICC detected inH9C2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 7 publications below

Product Information

68300-1-Ig targets Cardiac Troponin T in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.

Tested Reactivity human, mouse, rat, pig, rabbit, chicken
Cited Reactivityhuman, mouse, rat, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cardiac Troponin T fusion protein Ag7716
Full Name troponin T type 2 (cardiac)
Calculated Molecular Weight 36 kDa
Observed Molecular Weight34-40 kDa
GenBank Accession NumberBC002653
Gene Symbol Cardiac Troponin T
Gene ID (NCBI) 7139
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cardiac Troponin T, also named as cTnT, TNNT2. This gene encodes the cardiac isoform of troponin T. The encoded protein is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Cardiac Troponin T can be detected as about 34-40 kDa.

Protocols

Product Specific Protocols
WB protocol for Cardiac Troponin T antibody 68300-1-IgDownload protocol
IHC protocol for Cardiac Troponin T antibody 68300-1-IgDownload protocol
IF protocol for Cardiac Troponin T antibody 68300-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
rabbitIF

Adv Mater

DNA Framework-Enabled Three Dimensional Organization of Antiarrhythmic Drugs for Radiofrequency Catheter Ablation

Authors - Hangwei Chen
ratIF

Biochem Pharmacol

Trimetazidine attenuates Ischemia/Reperfusion-Induced myocardial ferroptosis by modulating the Sirt3/Nrf2-GSH system and reducing Oxidative/Nitrative stress

Authors - Mingyue Tan
mouseIF

J Inflamm Res

Cardamonin Attenuates Myocardial Ischemia/Reperfusion-Induced Ferroptosis Through Promoting STAT3 Signaling

Authors - Tao Yang
mouseIF

Clin Exp Hypertens

Exploration and validation of signature genes and immune associations in septic cardiomyopathy

Authors - Zhengbo Zhao
ratIF

J Transl Int Med

WWP2 protects against sepsis-induced cardiac injury through inhibiting cardiomyocyte ferroptosis

Authors - Zhi Li
ratIF

Toxicol Lett

Roles of AhR/CYP1s Signal Pathway Mediated ROS Production in Uremic Cardiomyopathy

Authors - Wei Lu