Cardiac Troponin T Monoclonal antibody

Cardiac Troponin T Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

mouse, rat, pig, rabbit, chicken and More (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Publications

6

CloneNo.

4E2D8

Cat no : 68300-1-Ig

Synonyms

TNNT2, cTnT, Troponin T, cardiac muscle, 4E2D8

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Validation Data Gallery

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  WB analysis of pig heart using 68300-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 68300-1-Ig (cTnT antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit heart using 68300-1-Ig

  rabbit heart tissue were subjected to SDS PAGE followed by western blot with 68300-1-Ig (cTnT antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 68300-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 68300-1-Ig (cTnT antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 68300-1-Ig

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 68300-1-Ig (cTnT antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of chicken heart using 68300-1-Ig

  chicken heart tissue were subjected to SDS PAGE followed by western blot with 68300-1-Ig (cTnT antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat heart using 68300-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 68300-1-Ig (Cardiac Troponin T antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 68300-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using cTnT antibody (68300-1-Ig, Clone: 4E2D8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of H9C2 using 68300-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using Cardiac Troponin T antibody (68300-1-Ig, Clone: 4E2D8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: rat heart tissue, pig heart tissue, rabbit heart tissue, mouse heart tissue, chicken heart tissue
• Positive IHC detected in: rat heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse heart tissue
• Positive IF/ICC detected in: H9C2 cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochemistry (IHC): IHC : 1:4000-1:16000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 6 publications below

Product Information

68300-1-Ig targets Cardiac Troponin T in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with mouse, rat, pig, rabbit, chicken samples.

• Tested Reactivity: mouse, rat, pig, rabbit, chicken
• Cited Reactivity: human, mouse, rat, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cardiac Troponin T fusion protein Ag7716
• Full Name: troponin T type 2 (cardiac)
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 34-40 kDa
• GenBank Accession Number: BC002653
• Gene Symbol: Cardiac Troponin T
• Gene ID (NCBI): 7139
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cardiac Troponin T, also named as cTnT, TNNT2. This gene encodes the cardiac isoform of troponin T. The encoded protein is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Cardiac Troponin T can be detected as about 34-40 kDa.

Publications

Species	Application	Title
rabbit	IF	Adv Mater; DNA Framework-Enabled Three Dimensional Organization of Antiarrhythmic Drugs for Radiofrequency Catheter Ablation; Authors - Hangwei Chen
rat	IF	Biochem Pharmacol; Trimetazidine attenuates Ischemia/Reperfusion-Induced myocardial ferroptosis by modulating the Sirt3/Nrf2-GSH system and reducing Oxidative/Nitrative stress; Authors - Mingyue Tan
mouse	IF	Clin Exp Hypertens; Exploration and validation of signature genes and immune associations in septic cardiomyopathy; Authors - Zhengbo Zhao
rat	IF	J Transl Int Med; WWP2 protects against sepsis-induced cardiac injury through inhibiting cardiomyocyte ferroptosis; Authors - Zhi Li
rat	IF	Toxicol Lett; Roles of AhR/CYP1s Signal Pathway Mediated ROS Production in Uremic Cardiomyopathy; Authors - Wei Lu
human	IF	Eur J Pharmacol; Forskolin is an effective therapeutic small molecule for the treatment of hypertrophic cardiomyopathy through ADCY6/cAMP/PKA pathway; Authors - Wenyan Wang