CoraLite® Plus 488-conjugated Calbindin-D28k Monoclonal antibody

Calbindin-D28k Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F8B9

Cat no : CL488-66394

Synonyms

Calbindin, CALB1, Calbindin D28, calbindin 1, 28kDa, CALB

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Validation Data Gallery

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  IF Staining of mouse cerebellum using CL488-66394

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using CoraLite® Plus 488 Calbindin-D28k antibody (CL488-66394, Clone: 1F8B9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse cerebellum using CL488-66394

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using CoraLite® Plus 488 Calbindin-D28k antibody (CL488-66394, Clone: 1F8B9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse cerebellum using CL488-66394

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using CoraLite® Plus 488 Calbindin-D28k antibody (CL488-66394, Clone: 1F8B9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse cerebellum using CL488-66394

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using CoraLite® Plus 488 Calbindin-D28k antibody (CL488-66394, Clone: 1F8B9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat cerebellum using CL488-66394

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat cerebellum tissue using CoraLite® Plus 488 Calbindin-D28k antibody (CL488-66394, Clone: 1F8B9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat cerebellum using CL488-66394

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat cerebellum tissue using CoraLite® Plus 488 Calbindin-D28k antibody (CL488-66394, Clone: 1F8B9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IF-P detected in: mouse cerebellum tissue, rat cerebellum tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66394 targets Calbindin-D28k in IF-P applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Calbindin-D28k fusion protein Ag5861
• Full Name: calbindin 1, 28kDa
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC006478
• Gene Symbol: Calbindin
• Gene ID (NCBI): 793
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Calbindin-D28k is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.