CaMKII Gamma Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CaMKII Gamma Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12666-2-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of SMMC-7721 using 12666-2-AP (same clone as 12666-2-PBS)

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 12666-2-AP (CaMKII gamma antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

• 

  WB analysis of SMMC-7721 using 12666-2-AP (same clone as 12666-2-PBS)

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 12666-2-AP (CaMKII gamma antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 12666-2-AP (same clone as 12666-2-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12666-2-AP (CaMKII gamma antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

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  IP experiment of mouse heart using 12666-2-AP (same clone as 12666-2-PBS)

  IP result of anti-CaMKII Gamma (IP:12666-2-AP, 4ug; Detection:12666-2-AP 1:1000) with mouse heart tissue lysate 3200ug. This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 12666-2-AP (same clone as 12666-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12666-2-AP (CaMKII Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 12666-2-AP (same clone as 12666-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12666-2-AP (CaMKII gamma antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 12666-2-AP (same clone as 12666-2-PBS)

  Immunofluorescent analysis of HepG2 cells, using CAMK2G antibody 12666-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

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Product Information

12666-2-PBS targets CaMKII Gamma in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CaMKII Gamma fusion protein Ag3348
• Full Name: calcium/calmodulin-dependent protein kinase II gamma
• Calculated Molecular Weight: 527 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC034044
• Gene Symbol: CAMK2G
• Gene ID (NCBI): 818
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CaMKII gamma, also named CAMKG or CAMK2G, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and CaMK subfamily. CAMK2 is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CAMK2G is expressed in human beta cells as a candidate gene for Type II (non-insulin-dependent) diabetes mellitus. Some studies can be used to assess the role of CAMK2G in the susceptibility to Type II diabetes(PMID: 12032636). CAMK2G has some isoforms with the MW of 55-66 kDa and this antibody can recognize all the isoforms of CAMK2G. This antibody may have cross-reaction with CAMK2A/B/D due to the high homology.