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Tested Applications
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-12286 targets CYP4F11 in IF/ICC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CYP4F11 fusion protein Ag2906 Predict reactive species |
| Full Name | cytochrome P450, family 4, subfamily F, polypeptide 11 |
| Calculated Molecular Weight | 524 aa, 60 kDa |
| Observed Molecular Weight | 57 kDa |
| GenBank Accession Number | BC016853 |
| Gene Symbol | CYP4F11 |
| Gene ID (NCBI) | 57834 |
| RRID | AB_3672532 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HBI6 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CYP4F11, also named as CYPIVF11, belongs to the cytochrome P450 family. It is the lone CYP4F/A enzyme that effectively oxidized 3-hydroxypalmitate. It is the predominant catalyst of this fatty acid oxidation reaction in human liver. CYP4F11 is one of the primary human epidermal keratinocytes. It is positively regulated by multiple signaling pathways in HaCaT keratinocytes, including RXR and JNK signaling pathways. (PMID:19812349)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CYP4F11 antibody CL488-12286 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
