CYP2R1 Recombinant antibody, PBS Only (Detector)

CYP2R1 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241072F11

Cat no : 84378-5-PBS

Synonyms

Vitamin D 25-hydroxylase, Vitamin D 25 hydroxylase, EC:1.14.14.24, 241072F11

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Validation Data Gallery

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  WB analysis using 84378-5-RR (same clone as 84378-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84378-5-RR (CYP2R1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84378-5-PBS in a different storage buffer formulation.

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  FC experiment of Hela using 84378-5-RR (same clone as 84378-5-PBS)

  1x10^6 Hela cells were intracellularly stained with 0.25 ug CYP2R1 Recombinant antibody (84378-5-RR, Clone:241072F11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84378-5-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01251-2

  Cytometric bead array standard curve of MP01251-2, CYP2R1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84378-3-PBS. Detection antibody: 84378-5-PBS. Standard: Ag12873. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84378-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84378-5-RR against Human CYP2R1 were performed. The affinity constant is 0.243 nM.

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Product Information

84378-5-PBS targets CYP2R1 as part of a matched antibody pair:

MP01251-2: 84378-3-PBS capture and 84378-5-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CYP2R1 fusion protein Ag12873
• Full Name: cytochrome P450, family 2, subfamily R, polypeptide 1
• Calculated Molecular Weight: 501 aa, 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC104909
• Gene Symbol: CYP2R1
• Gene ID (NCBI): 120227
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CYP2R1 is a member of the cytochrome P450 superfamily of enzymes which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids, and other lipids. CYP2R1 primarily functions as a 25-hydroxylase of vitamin D, responsible for converting vitamin D to 25-hydroxyvitamin D (25-OH-D), affecting the production of the active hormone 1,25(OH)2D (PMID: 12867411). CYP2R1 enzyme is a key vitamin D 25-hydroxylase, mutations in CYP2R1 are associated with Rickets vitamin D-dependent 1B(PMID: 25942481, 15128933).