CYP20A1 Monoclonal antibody, PBS Only

CYP20A1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A9E2

Cat no : 67433-1-PBS

Synonyms

CYP M, CYP20A1, Cytochrome P450 20A1

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Validation Data Gallery

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  WB analysis using 67433-1-Ig (same clone as 67433-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67433-1-Ig (CYP20A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67433-1-Ig (same clone as 67433-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67433-1-Ig (CYP20A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67433-1-Ig (same clone as 67433-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67433-1-Ig (CYP20A1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67433-1-Ig (same clone as 67433-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67433-1-Ig (CYP20A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67433-1-Ig (same clone as 67433-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67433-1-Ig (CYP20A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67433-1-Ig (same clone as 67433-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CYP20A1 antibody (67433-1-Ig, Clone: 2A9E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67433-1-Ig (same clone as 67433-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CYP20A1 antibody (67433-1-Ig, Clone: 2A9E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

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Product Information

67433-1-PBS targets CYP20A1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CYP20A1 fusion protein Ag9715
• Full Name: cytochrome P450, family 20, subfamily A, polypeptide 1
• Calculated Molecular Weight: 462 aa, 52 kDa
• Observed Molecular Weight: 49-52 kDa
• GenBank Accession Number: BC020616
• Gene Symbol: CYP20A1
• Gene ID (NCBI): 57404
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.