CYP1B1 Monoclonal antibody, PBS Only

CYP1B1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G8D4

Cat no : 67033-1-PBS

Synonyms

GLC3A, EC:4.2.1.152, EC:1.14.14.1, CYPIB1, CP1B

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Validation Data Gallery

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  WB analysis using 67033-1-Ig (same clone as 67033-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67033-1-Ig (CYP1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 67033-1-Ig (same clone as 67033-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 67033-1-Ig (CYP1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 67033-1-Ig (same clone as 67033-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67033-1-Ig (CYP1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 67033-1-Ig (same clone as 67033-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67033-1-Ig (CYP1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 67033-1-Ig (same clone as 67033-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67033-1-Ig (CYP1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 67033-1-Ig (same clone as 67033-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67033-1-Ig (CYP1B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 67033-1-Ig (same clone as 67033-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67033-1-Ig (CYP1B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67033-1-Ig (same clone as 67033-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67033-1-Ig (CYP1B1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67033-1-Ig (same clone as 67033-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67033-1-Ig (CYP1B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 67033-1-Ig (same clone as 67033-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 67033-1-Ig (CYP1B1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 67033-1-Ig (same clone as 67033-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 67033-1-Ig (CYP1B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67033-1-PBS in a different storage buffer formulation.

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Product Information

67033-1-PBS targets CYP1B1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CYP1B1 fusion protein Ag13380
• Full Name: cytochrome P450, family 1, subfamily B, polypeptide 1
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC012049
• Gene Symbol: CYP1B1
• Gene ID (NCBI): 1545
• RRID: AB_2882348
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Cytochrome P450 CYP1B1 is a recently cloned dioxin-inducible form of the cytochrome P450 family of xenobiotic metabolizing enzymes. CYP1B1 was found to be expressed at a high frequency in a wide range of human cancers of different histogenetic types, including cancers of the breast, colon, lung, esophagus, skin, lymph node, brain, and testis. There was no detectable immunostaining for CYP1B1 in normal tissues.(PMID:9230218). CYP1B1 is a 60 kDa protein that it also can be detected a band of 51 kDa or 57 kDa through the western blot(PMID:9525272; PMID: 9525272).