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CYP1B1 Monoclonal antibody

CYP1B1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G8D4

Cat no : 67033-1-Ig

Synonyms

GLC3A, EC:4.2.1.152, EC:1.14.14.1, CYPIB1, CP1B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CYP1B1 Monoclonal antibody (67033-1-Ig)

    WB analysis using 67033-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67033-1-Ig (CYP1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using CYP1B1 Monoclonal antibody (67033-1-Ig)

    WB analysis of PC-3 using 67033-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 67033-1-Ig (CYP1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using CYP1B1 Monoclonal antibody (67033-1-Ig)

    WB analysis of pig brain using 67033-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67033-1-Ig (CYP1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using CYP1B1 Monoclonal antibody (67033-1-Ig)

    WB analysis of rat brain using 67033-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67033-1-Ig (CYP1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CYP1B1 Monoclonal antibody (67033-1-Ig)

    WB analysis of mouse brain using 67033-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67033-1-Ig (CYP1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using CYP1B1 Monoclonal antibody (67033-1-Ig)

    IHC staining of mouse heart using 67033-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67033-1-Ig (CYP1B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using CYP1B1 Monoclonal antibody (67033-1-Ig)

    IHC staining of mouse heart using 67033-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67033-1-Ig (CYP1B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CYP1B1 Monoclonal antibody (67033-1-Ig)

    IHC staining of mouse brain using 67033-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67033-1-Ig (CYP1B1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CYP1B1 Monoclonal antibody (67033-1-Ig)

    IHC staining of mouse brain using 67033-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67033-1-Ig (CYP1B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using CYP1B1 Monoclonal antibody (67033-1-Ig)

    IHC staining of human rectal cancer using 67033-1-Ig

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 67033-1-Ig (CYP1B1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using CYP1B1 Monoclonal antibody (67033-1-Ig)

    IHC staining of human rectal cancer using 67033-1-Ig

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 67033-1-Ig (CYP1B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, PC-3 cells, rat brain tissue, mouse brain tissue, pig brain tissue, T-47D cells, MCF-7 cells
Positive IHC detected inmouse heart tissue, human rectal cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67033-1-Ig targets CYP1B1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CYP1B1 fusion protein Ag13380
Full Name cytochrome P450, family 1, subfamily B, polypeptide 1
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC012049
Gene Symbol CYP1B1
Gene ID (NCBI) 1545
RRIDAB_2882348
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytochrome P450 CYP1B1 is a recently cloned dioxin-inducible form of the cytochrome P450 family of xenobiotic metabolizing enzymes. CYP1B1 was found to be expressed at a high frequency in a wide range of human cancers of different histogenetic types, including cancers of the breast, colon, lung, esophagus, skin, lymph node, brain, and testis. There was no detectable immunostaining for CYP1B1 in normal tissues.(PMID:9230218). CYP1B1 is a 60 kDa protein that it also can be detected a band of 51 kDa or 57 kDa through the western blot(PMID:9525272; PMID: 9525272).

Protocols

Product Specific Protocols
WB protocol for CYP1B1 antibody 67033-1-IgDownload protocol
IHC protocol for CYP1B1 antibody 67033-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols