CYLD Monoclonal antibody, PBS Only

CYLD Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G2F4

Cat no : 66858-1-PBS

Synonyms

CDMT, CYLD, CYLD1, CYLDI, Deubiquitinating enzyme CYLD, EAC, FLJ20180, FLJ31664, FLJ78684, HSPC057, KIAA0849, MFT, MFT1, SBS, TEM, Ubiquitin thiolesterase CYLD, USPL2

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Validation Data Gallery

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  WB analysis using 66858-1-Ig (same clone as 66858-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66858-1-Ig (CYLD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66858-1-PBS in a different storage buffer formulation.

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  WB analysis using 66858-1-Ig (same clone as 66858-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66858-1-Ig (CYLD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66858-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66858-1-Ig (same clone as 66858-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66858-1-Ig (CYLD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66858-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 66858-1-Ig (same clone as 66858-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66858-1-Ig (CYLD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66858-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 66858-1-Ig (same clone as 66858-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CYLD antibody (66858-1-Ig, Clone: 1G2F4 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66858-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 66858-1-Ig (same clone as 66858-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CYLD antibody (66858-1-Ig, Clone: 1G2F4 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66858-1-PBS in a different storage buffer formulation.

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Product Information

66858-1-PBS targets CYLD in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: Human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CYLD fusion protein Ag28333
• Full Name: cylindromatosis (turban tumor syndrome)
• Calculated Molecular Weight: 107 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC012342
• Gene Symbol: CYLD
• Gene ID (NCBI): 1540
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CYLD, also named as CYLD1, belongs to the peptidase C67 family. It is the protease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. CYLD has endodeubiquitinase activity and plays an important role in the regulation of pathways leading to NF-kappa-B activation. CYLD contributes to the regulation of cell survival, proliferation and differentiation via its effects on NF-kappa-B activation. It is a negative regulator of Wnt signaling. CYLD inhibits HDAC6 and thereby promotes acetylation of alpha-tubulin and stabilization of microtubules. CYLD plays a role in the regulation of microtubule dynamics, and thereby contributes to the regulation of cell proliferation, cell polarization, cell migration, and angiogenesis. It is required for normal cell cycle progress and normal cytokinesis. CYLD inhibits nuclear translocation of NF-kappa-B and plays a role in the regulation of inflammation and the innate immune response, via its effects on NF-kappa-B activation. It is dispensable for the maturation of intrathymic natural killer cells, but required for the continued survival of immature natural killer cells. CYLD negatively regulates TNFRSF11A signaling and osteoclastogenesis.