CYLD Monoclonal antibody

CYLD Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1G2F4

Cat no : 66858-1-Ig

Synonyms

CDMT, CYLD, CYLD1, CYLDI, Deubiquitinating enzyme CYLD, EAC, FLJ20180, FLJ31664, FLJ78684, HSPC057, KIAA0849, MFT, MFT1, SBS, TEM, Ubiquitin thiolesterase CYLD, USPL2

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Validation Data Gallery

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  WB analysis using 66858-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66858-1-Ig (CYLD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis using 66858-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66858-1-Ig (CYLD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66858-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66858-1-Ig (CYLD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 66858-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66858-1-Ig (CYLD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of SH-SY5Y using 66858-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CYLD antibody (66858-1-Ig, Clone: 1G2F4 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of SH-SY5Y using 66858-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CYLD antibody (66858-1-Ig, Clone: 1G2F4 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: pig brain tissue, HEK-293 cells, A431 cells, rat brain tissue, mouse brain tissue, rabbit brain tissue
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66858-1-Ig targets CYLD in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: Human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CYLD fusion protein Ag28333
• Full Name: cylindromatosis (turban tumor syndrome)
• Calculated Molecular Weight: 107 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC012342
• Gene Symbol: CYLD
• Gene ID (NCBI): 1540
• RRID: AB_2882197
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYLD, also named as CYLD1, belongs to the peptidase C67 family. It is the protease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. CYLD has endodeubiquitinase activity and plays an important role in the regulation of pathways leading to NF-kappa-B activation. CYLD contributes to the regulation of cell survival, proliferation and differentiation via its effects on NF-kappa-B activation. It is a negative regulator of Wnt signaling. CYLD inhibits HDAC6 and thereby promotes acetylation of alpha-tubulin and stabilization of microtubules. CYLD plays a role in the regulation of microtubule dynamics, and thereby contributes to the regulation of cell proliferation, cell polarization, cell migration, and angiogenesis. It is required for normal cell cycle progress and normal cytokinesis. CYLD inhibits nuclear translocation of NF-kappa-B and plays a role in the regulation of inflammation and the innate immune response, via its effects on NF-kappa-B activation. It is dispensable for the maturation of intrathymic natural killer cells, but required for the continued survival of immature natural killer cells. CYLD negatively regulates TNFRSF11A signaling and osteoclastogenesis.