CoraLite®594-conjugated CYB5R3 Polyclonal antibody

• Featured Product
• KD/KO Validated

CYB5R3 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-10894

Synonyms

NADH-cytochrome b5 reductase 3, EC:1.6.2.2, Diaphorase-1, Diaphorase 1, DIA1

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Validation Data Gallery

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  IF Staining of HeLa using CL594-10894

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 CYB5R3 antibody (CL594-10894) at dilution of 1:200.

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  FC experiment of HeLa using CL594-10894

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CYB5R3 (CL594-10894) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-10894 targets CYB5R3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYB5R3 fusion protein Ag1339
• Full Name: cytochrome b5 reductase 3
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC004821
• Gene Symbol: CYB5R3
• Gene ID (NCBI): 1727
• RRID: AB_2919751
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CYB5R3, also named as DIA1 and B5R, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. It plays an essential role in the mitochondrial metabolism by its contribution to cellular redox homeostasis. Overstimulation of CYB5R3 associated with lipid rafts can account for the overshot of plasma membrane-focalized superoxide anion production that triggers the entry of CGN in the irreversible phase of apoptosis.