CoraLite® Plus 488-conjugated CXCR7 Monoclonal antibody

CXCR7 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4C3D7

Cat no : CL488-60216

Synonyms

ACKR3, Chemokine orphan receptor 1, CMKOR1, CXC R7, CXCR 7, CXCR7, G protein coupled receptor 159, GPR159, RDC 1, RDC1

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Validation Data Gallery

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  IF Staining of SW480 using CL488-60216

  Immunofluorescent analysis of (-20°C Methanol) fixed SW480 cells using CoraLite® Plus 488 CXCR7 antibody (CL488-60216, Clone: 4C3D7 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: SW480 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60216 targets CXCR7 in IF/ICC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CXCR7 fusion protein Ag14247
• Full Name: chemokine (C-X-C motif) receptor 7
• Calculated Molecular Weight: 362 aa, 41 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC036661
• Gene Symbol: CXCR7
• Gene ID (NCBI): 57007
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CXCR7 (C-X-C chemokine receptor type 7), also known as RDC1, is a member of the G-protein coupled receptor family. CXCR7 can bind the chemokines CXCL11 and CXCL12 with high affinity, and it also acts as coreceptor with CXCR4 for a restricted number of HIV isolates. Expression of CXCR7 has been associated with cardiac development as well as with tumor growth and progression. This antibody recognizes endogenous CXCR7, which has an experimentally determined molecular weight of 50 kDa (PMID: 20197403; 20388803).