CXCR7 Monoclonal antibody, PBS Only

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  WB analysis using 60216-1-Ig (same clone as 60216-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60216-1-Ig (CXCR7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60216-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60216-1-Ig (same clone as 60216-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60216-1-Ig (CXCR7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60216-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60216-1-Ig (same clone as 60216-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60216-1-Ig (CXCR7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60216-1-PBS in a different storage buffer formulation.

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  IF Staining of human prostate cancer using 60216-1-Ig (same clone as 60216-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CXCR7 antibody (60216-1-Ig, Clone: 4C3D7 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60216-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 60216-1-Ig (same clone as 60216-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CXCR7 antibody (60216-1-Ig, Clone: 4C3D7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60216-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 60216-1-Ig (same clone as 60216-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CXCR7 antibody (60216-1-Ig, Clone: 4C3D7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60216-1-PBS in a different storage buffer formulation.

CXCR7 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 60216-1-PBS

Clone No.4C3D7

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

ACKR3, CXC R7, C-X-C chemokine receptor type 7, CMKOR1, Chemokine orphan receptor 1

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Product Information

60216-1-PBS targets CXCR7 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CXCR7 fusion protein Ag14247
• Full Name: chemokine (C-X-C motif) receptor 7
• Calculated Molecular Weight: 362 aa, 41 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC036661
• Gene Symbol: CXCR7
• Gene ID (NCBI): 57007
• RRID: AB_11182925
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P25106
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

CXCR7 (C-X-C chemokine receptor type 7), also known as RDC1, is a member of the G-protein coupled receptor family. CXCR7 can bind the chemokines CXCL11 and CXCL12 with high affinity, and it also acts as a coreceptor with CXCR4 for a restricted number of HIV isolates. Expression of CXCR7 has been associated with cardiac development as well as with tumor growth and progression. This antibody recognizes endogenous CXCR7, which has an experimentally determined molecular weight of 50 kDa (PMID: 20197403; 20388803).