Anti-Human CXCR4/CD184 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using 98175-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CXCR4/CD184 Rabbit Recombinant Antibody (98175-1-RR, Clone: 241735F8),or Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 98175-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CXCR4/CD184 Rabbit Recombinant Antibody (98175-1-RR, Clone:241735F8)(red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

CXCR4/CD184 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98175-1-RR

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CXCR4, CXCR 4, CXC R4, C-X-C chemokine receptor type 4, CD184

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98175-1-RR targets CXCR4/CD184 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: chemokine (C-X-C motif) receptor 4
• Calculated Molecular Weight: 352 aa, 40 kDa
• GenBank Accession Number: BC020968
• Gene Symbol: CXCR4
• Gene ID (NCBI): 7852
• RRID: AB_3672318
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P61073
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

C-X-C chemokine receptor type 4 (CXCR4, also known as CD184) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609).