Biotin-conjugated CXCR4 Monoclonal antibody

• Featured Product
• KD/KO Validated

CXCR4 Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgM

Reactivity

human, mouse

Applications

IHC

Conjugate

Biotin

CloneNo.

4B5E4

Cat no : Biotin-60042

Synonyms

CD184, CXC R4, CXCR 4, CXCR4, CXCR4B, D2S201E, FB22, Fusin, HM89, HSY3RR, LAP3, LCR1, LESTR, SDF 1 receptor, WHIM

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Validation Data Gallery

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  IHC staining of human breast cancer using Biotin-60042

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using Biotin-60042 (CXCR4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using Biotin-60042

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using Biotin-60042 (CXCR4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using Biotin-60042

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using Biotin-60042 (CXCR4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using Biotin-60042

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using Biotin-60042 (CXCR4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using Biotin-60042

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-60042 (CXCR4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using Biotin-60042

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-60042 (CXCR4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human breast cancer tissue, mouse spleen tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-60042 targets CXCR4 in IHC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: CXCR4 fusion protein Ag1528
• Full Name: chemokine (C-X-C motif) receptor 4
• Calculated Molecular Weight: 352 aa, 40 kDa
• Observed Molecular Weight: 60~70 kDa
• GenBank Accession Number: BC020968
• Gene Symbol: CXCR4
• Gene ID (NCBI): 7852
• RRID: AB_2918944
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Caprylic acid/ammonium sulfate precipitation
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

C-X-C chemokine receptor type 4 (CXCR4) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609). CXCR4 has a calculated molecular weight of 40 kDa. In addition to the predicted species of 45-47 kDa for glycosylated CXCR4 monomers, due to ubiquitination, dimeration, and different degrees of glycosylation, additional species can also exist and have been reported in some researches, including 67 kDa (PMID: 23917520), 55, 67, 87 kDa (PMID: 20028517), 80 kDa (PMID: 10506573), 47, 50, 62, and 98 kDa (PMID: 16204649).