CXCL8,IL8 Recombinant antibody, PBS Only (Capture)

CXCL8,IL8 Uni-rAb^TM Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240889B4

Cat no : 83903-5-PBS

Synonyms

CXCL8, IL8, IL-8, C-X-C motif chemokine 8, C-X-C motif

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00830-3

  Sandwich ELISA standard curve of MP00830-3, Human CXCL8/IL-8 Recombinant Matched Antibody Pair - PBS only. 83903-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0152. 83903-1-PBS was HRP conjugated as the detection antibody. Range: 2.73-175 pg/mL.

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  Sample test of MP00830-3

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 or 5 days. For the PBMC supernatant cultured for 3 days, the mean CXCL8/IL-8 concentration was determined to be 4.46 ng/mL in unstimulated PBMC supernatant, 252.88 ng/mL in PHA stimulated PBMC supernatant. For the PBMC supernatant cultured for 5 days, the mean CXCL8/IL-8 concentration was determined to be 317.16 ng/mL in PHA stimulated PBMC supernatant.

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  Sample test of MP00830-3

  Serum of sixteen individual healthy human donors was measured. The CXCL8/IL-8 concentration of detected samples was determined to be 29.8 pg/mL with a range of 8.9 - 104.1 pg/mL.

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Product Information

83903-5-PBS targets CXCL8,IL8 as part of a matched antibody pair:

MP00830-3: 83903-5-PBS capture and 83903-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: interleukin 8
• Calculated Molecular Weight: 99 aa, 11 kDa
• GenBank Accession Number: BC013615
• Gene Symbol: IL-8
• Gene ID (NCBI): 3576
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.