Anti-Human CXCL8/IL-8 Rabbit Recombinant Antibody, PBS Only

CXCL8/IL-8 Uni-rAb^TM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

240889B2

Cat no : 98137-1-PBS

Synonyms

CXCL8, IL8, IL-8, C-X-C motif chemokine 8, C-X-C motif

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Validation Data Gallery

• 

  FC experiment of human PBMCs using 98137-1-RR (same clone as 98137-1-PBS)

  1x10^6 untreated or IFN gamma, LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL8/IL-8 Rabbit Recombinant Antibody (98137-1-RR, Clone: 240889B2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Granulocytes were gated. This data was developed using the same antibody clone with 98137-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98137-1-RR (same clone as 98137-1-PBS)

  1x10^6 IFN gamma, LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL8/IL-8 Rabbit Recombinant Antibody (98137-1-RR, Clone: 240889B2) (red) or Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 unstimulated human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL8/IL-8 Rabbit Recombinant Antibody (98137-1-RR, Clone: 240889B2) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Granulocytes were gated. This data was developed using the same antibody clone with 98137-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98137-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98137-1-RR against Human CXCL8/IL-8 were performed. The affinity constant is below 1 pM.

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Product Information

The immunogen of 98137-1-PBS is CXCL8/IL-8 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: interleukin 8
• Calculated Molecular Weight: 99 aa, 11 kDa
• GenBank Accession Number: BC013615
• Gene Symbol: IL-8
• Gene ID (NCBI): 3576
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Interleukin 8 (IL-8), also known as CXCL8, is a CXC chemokine family member. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli. IL-8 has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also induces phagocytosis once they have arrived. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. IL-8 is also known to be a potent promoter of angiogenesis. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression.