CXCL1 Recombinant antibody, PBS Only (Capture/Detector)

CXCL1 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240990E5

Cat no : 83937-1-PBS

Synonyms

GRO-alpha(5-73), GRO-alpha(4-73), CXCL 1, C-X-C motif chemokine 1, C X C motif chemokine 1

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00922-2

  Sandwich ELISA standard curve of MP00922-2, Human CXCL1 Recombinant Matched Antibody Pair - PBS only. 83937-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2917. 83937-2-PBS was biotinylated as the detection antibody. Range: 31.25-2000 pg/mL

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  Sample test of MP00922-2

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 5 days. The mean CXCL1 concentration was determined to be 32.4 pg/mL in unstimulated PBMC supernatant, 9,728.4 pg/mL in PHA stimulated PBMC supernatant.

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  Sample test of MP00922-2

  Serum of tweleve individual healthy human donors was measured. The human CXCL1 concentration of detected samples was determined to be 110.0 pg/mL with a range of 0.6 - 1,164.0 pg/mL.

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  Cytometric bead array standard curve of MP00922-1

  Cytometric bead array standard curve of MP00922-1, CXCL1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83937-2-PBS. Detection antibody: 83937-1-PBS. Standard: Ag2917. Range: 0.78-100 ng/mL.

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Product Information

83937-1-PBS targets CXCL1 as part of a matched antibody pair:

MP00922-1: 83937-2-PBS capture and 83937-1-PBS detection (validated in Cytometric bead array)

MP00922-2: 83937-1-PBS capture and 83937-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CXCL1 fusion protein Ag2917
• Full Name: chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
• Calculated Molecular Weight: 8 kDa to 14 kDa
• GenBank Accession Number: BC011976
• Gene Symbol: CXCL1
• Gene ID (NCBI): 2919
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CXCL1 a is a member of CXC family and also known as keratinocyte-derived chemokines (KC) or growth-related oncogene (GRO). CXCL1 is expressed by macrophages, neutrophils and epithelial cells. CXCL1 binding specifically to the CXC chemokine receptor CXCR2, is involved in fibrogenesis and angiogenesis. This protein also plays a role in inflammation and as a chemoattractant for neutrophils. CXCL1 is upregulated in some types of human cancer, including colorectal, bladder, breast, prostate and skin cancers.