CoraLite®594-conjugated CUL4A Monoclonal antibody

CUL4A Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, monkey, mouse, rat, pig

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A7F12

Cat no : CL594-66038

Synonyms

CUL 4A, CUL4A, cullin 4A

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-66038

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66038 (CUL4A antibody) at dilution of 1:100.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL594-66038 is CUL4A Fusion Protein expressed in E. coli.

• Tested Reactivity: human, monkey, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CUL4A fusion protein Ag18089
• Full Name: cullin 4A
• Calculated Molecular Weight: 77 kDa
• Observed Molecular Weight: 77 kDa, 88 kDa
• GenBank Accession Number: BC008308
• Gene Symbol: CUL4A
• Gene ID (NCBI): 8451
• RRID: AB_2883486
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Cullin proteins assemble a large number of RING E3 ubiquitin ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), have been identified. The two CUL4 sequences are 83% identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases, DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol, suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2.