CUL3 Recombinant antibody, PBS Only (Capture/Detector)

CUL3 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240534A6

Cat no : 83619-1-PBS

Synonyms

KIAA0617, Cullin-3, CUL-3, 240534A6

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00599-4

  Sandwich ELISA standard curve of MP00599-4, Human CUL3 Recombinant Matched Antibody Pair - PBS only. 83619-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1555. 83619-1-PBS was HRP conjugated as the detection antibody. Range: 1.25-80 ng/mL

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  Sample test of MP00599-4

  Hela and Jurkat cell lysates were measured. The human CUL3 concentration of detected samples was determined to be 144.2 ng/mL (based on a 1.2 mg/mL extract load) in Hela cell lysate and 475.5 ng/mL (based on a 1.0 mg/mL extract load) in Jurkat cell lysate.

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  Cytometric bead array standard curve of MP00599-1

  Cytometric bead array standard curve of MP00599-1, CUL3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83619-1-PBS. Detection antibody: 83619-2-PBS. Standard: Ag1555. Range: 0.625-80 ng/mL.

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  Cytometric bead array standard curve of MP00599-2

  Cytometric bead array standard curve of MP00599-2, CUL3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83619-4-PBS. Detection antibody: 83619-1-PBS. Standard: Ag1555. Range: 0.625-80 ng/mL.

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  Cytometric bead array standard curve of MP00599-3

  Cytometric bead array standard curve of MP00599-3, CUL3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83619-1-PBS. Detection antibody: 83619-3-PBS. Standard: Ag1555. Range: 0.625-80 ng/mL.

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Product Information

83619-1-PBS targets CUL3 as part of a matched antibody pair:

MP00599-1: 83619-1-PBS capture and 83619-2-PBS detection (validated in Cytometric bead array)

MP00599-2: 83619-4-PBS capture and 83619-1-PBS detection (validated in Cytometric bead array)

MP00599-3: 83619-1-PBS capture and 83619-3-PBS detection (validated in Cytometric bead array)

MP00599-4: 83619-7-PBS capture and 83619-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CUL3 fusion protein Ag1555
• Full Name: cullin 3
• Calculated Molecular Weight: 89 kDa
• GenBank Accession Number: BC039598
• Gene Symbol: CUL3
• Gene ID (NCBI): 8452
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.