CUL1 Monoclonal antibody, PBS Only

CUL1 Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B6D10

Cat no : 66978-1-PBS

Synonyms

CUL 1, CUL1, cullin 1

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Validation Data Gallery

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  WB analysis using 66978-1-Ig (same clone as 66978-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66978-1-Ig (CUL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66978-1-PBS in a different storage buffer formulation.

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  WB analysis using 66978-1-Ig (same clone as 66978-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66978-1-Ig (CUL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66978-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66978-1-Ig (same clone as 66978-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66978-1-Ig (CUL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66978-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66978-1-Ig (same clone as 66978-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66978-1-Ig (CUL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66978-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 66978-1-Ig (same clone as 66978-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CUL1 antibody (66978-1-Ig, Clone: 1B6D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66978-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 66978-1-Ig (same clone as 66978-1-PBS)

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human CUL1 (66978-1-Ig, Clone:1B6D10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66978-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66978-1-PBS targets CUL1 in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CUL1 fusion protein Ag28399
• Full Name: cullin 1
• Calculated Molecular Weight: 90 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC034318
• Gene Symbol: CUL1
• Gene ID (NCBI): 8454
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.