CUL1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CUL1 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

22

Reviews (1)

Cat no : 12895-1-AP

Synonyms

Cullin-1, Cullin1, CUL-1

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Validation Data Gallery

• 

  WB analysis using 12895-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12895-1-AP (CUL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 12895-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12895-1-AP (CUL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 12895-1-AP

  IP result of anti-CUL1 (IP:12895-1-AP, 4ug; Detection:12895-1-AP 1:2000) with K-562 cells lysate 1760 ug.

• 

  IP experiment of HeLa using 12895-1-AP

  IP result of anti-CUL1 (IP:12895-1-AP, 3ug; Detection:12895-1-AP 1:300) with HeLa cells lysate 2000ug.

• 

  IHC staining of human lung cancer using 12895-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12895-1-AP (CUL1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12895-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12895-1-AP (CUL1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HeLa using 12895-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CUL1 (12895-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: A549 cells, BxPC-3 cells, K-562 cells
• Positive IP detected in: K-562 cells, HeLa cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 22 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

12895-1-AP targets CUL1 in WB, IHC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CUL1 fusion protein Ag3585
• Full Name: cullin 1
• Calculated Molecular Weight: 90 kDa
• Observed Molecular Weight: 80-90 kDa
• GenBank Accession Number: BC034318
• Gene Symbol: CUL1
• Gene ID (NCBI): 8454
• RRID: AB_2086291
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.

Publications

Species	Application	Title
mouse	WB	Redox Biol; Cardioprotective effect of MLN4924 on ameliorating autophagic flux impairment in myocardial ischemia-reperfusion injury by Sirt1.; Authors - Ji Zhang
human	WB	Front Bioeng Biotechnol; Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts.; Authors - Kaile Zhang
mouse	WB,IP	Pharmacol Res; Cullin-associated and neddylation-dissociated 1 protein (CAND1) governs cardiac hypertrophy and heart failure partially through regulating calcineurin degradation.; Authors - Xingda Li
human	WB	iScience; A small-molecule Skp1 inhibitor elicits cell death by p53-dependent mechanism.; Authors - Muzammal Hussain
human	WB	J Cell Sci; Cullin-3-KCTD10-mediated CEP97 degradation promotes primary cilium formation.; Authors - Tomoaki Nagai ; KD Validated
human	WB	Cancer Cell Int; The potential oncogenic and MLN4924-resistant effects of CSN5 on cervical cancer cells.; Authors - Huilin Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

WEI (Verified Customer) (05-11-2022)

strong band for predicted band

• Applications: Western Blot
• Cell Tissue Type: Mouse Heart

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