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Cathepsin B Polyclonal antibody

Cathepsin B Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 12216-1-AP

Synonyms

APP secretase, APPS, cathepsin B, Cathepsin B1, CPSB, CTSB



Tested Applications

Positive WB detected inA375 cells, HepG2 cells, HT-1080 cells
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA375 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

12216-1-AP targets Cathepsin B in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, pig, frog
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cathepsin B fusion protein Ag2898
Full Name cathepsin B
Calculated molecular weight 339 aa, 38 kDa
Observed molecular weight 38 kDa, 44 kDa
GenBank accession numberBC010240
Gene symbol CTSB
Gene ID (NCBI) 1508
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTSB(Cathepsin B) is also named as CPSB and belongs to the peptidase C1 family. It participates in intracellular degradation and turnover of proteins. Cathepsin B precursors found in human malignant ascites fluid do not possess mannose-rich carbohydrates suggesting that a defect in the post translational processing of carbohydrate moieties on tumor. Cathepsin B exists as both glycosylated and unglycosylated forms(PMID:1637335). In rat macrophages and hepatocytes pro- cathepsin B is 39 kDa (unglycosylated =35 kDa), whereas in human fibroblasts procathepsin B is 44.5-46kDa (unglycosylated=39kDa)(PMID:2097084). It can be detected the 43 kDa form of pro-CTSB, 31 kDa and 25 kDa mature forms in mouse brain by western blot(PMID:20616152).

Protocols

Product Specific Protocols
WB protocol for Cathepsin B antibody 12216-1-APDownload protocol
IHC protocol for Cathepsin B antibody 12216-1-APDownload protocol
IF protocol for Cathepsin B antibody 12216-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Nature

Neurotoxic microglia promote TDP-43 proteinopathy in progranulin deficiency.

Authors - Jiasheng Zhang
mouseWB,IHC

Aging Cell

Growth differentiation factor 11 accelerates liver senescence through the inhibition of autophagy

Authors - Jian Sun
humanWB

Redox Biol

Inhibition of STAT3-ferroptosis negative regulatory axis suppresses tumor growth and alleviates chemoresistance in gastric cancer.

Authors - Shumin Ouyang
humanWB

Sci Total Environ

TFEB coordinates autophagy and pyroptosis as hepatotoxicity responses to ZnO nanoparticles

Authors - Xingyao Pei
frogWB

Proc Natl Acad Sci U S A

Host-derived, pore-forming toxin-like protein and trefoil factor complex protects the host against microbial infection.

Authors - Yang Xiang
mouseWB

Autophagy

The role of NLRP3-CASP1 in inflammasome-mediated neuroinflammation and autophagy dysfunction in manganese-induced, hippocampal-dependent impairment of learning and memory ability.

Authors - Diya Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Weihao (Verified Customer) (02-03-2022)

Works well for mouse BMDM and human macrophages, recommend it

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500