Cathepsin B Polyclonal antibody

• Featured Product
• KD/KO Validated

Cathepsin B Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (5)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

66

Reviews (1)

Cat no : 12216-1-AP

Synonyms

CTSB, Cathepsin B1, Cathepsin B light chain, Cathepsin B heavy chain, Cat B

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Validation Data Gallery

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  WB analysis using 12216-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12216-1-AP (Cathepsin B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12216-1-AP

  WB result of Cathepsin B antibody (12216-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cathepsin B transfected HepG2 cells.

• 

  IHC staining of human ovary tumor using 12216-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12216-1-AP (Cathepsin B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12216-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12216-1-AP (Cathepsin B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 12216-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12216-1-AP (Cathepsin B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A375 using 12216-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A375 cells using Cathepsin B antibody (12216-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of MCF-7 using 12216-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human Cathepsin B (12216-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A375 cells, HepG2 cells, HT-1080 cells
• Positive IHC detected in: human ovary tumor tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A375 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 50 publications below
• IHC: See 6 publications below
• IF: See 16 publications below

Product Information

12216-1-AP targets Cathepsin B in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, pig, bovine, frog
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cathepsin B fusion protein Ag2898
• Full Name: cathepsin B
• Calculated Molecular Weight: 339 aa, 38 kDa
• Observed Molecular Weight: 25 kDa, 31 kDa, 43 kDa
• GenBank Accession Number: BC010240
• Gene Symbol: Cathepsin B
• Gene ID (NCBI): 1508
• RRID: AB_2086929
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTSB(Cathepsin B) is also named as CPSB and belongs to the peptidase C1 family. It participates in intracellular degradation and turnover of proteins. Cathepsin B precursors found in human malignant ascites fluid do not possess mannose-rich carbohydrates suggesting that a defect in the post translational processing of carbohydrate moieties on tumor. Cathepsin B exists as both glycosylated and unglycosylated forms(PMID:1637335). In rat macrophages and hepatocytes pro- cathepsin B is 39 kDa (unglycosylated =35 kDa), whereas in human fibroblasts procathepsin B is 44.5-46kDa (unglycosylated=39kDa)(PMID:2097084). It can be detected the 43 kDa form of pro-CTSB, 31 kDa and 25 kDa mature forms in mouse brain by western blot(PMID:20616152).

Publications

Species	Application	Title
mouse	WB,IF	Nature; Neurotoxic microglia promote TDP-43 proteinopathy in progranulin deficiency.; Authors - Jiasheng Zhang
human	IF	Cell Death Differ; Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration; Authors - Huaizhen Liang
mouse	WB,IHC	Aging Cell; Growth differentiation factor 11 accelerates liver senescence through the inhibition of autophagy; Authors - Jian Sun
human	WB	Redox Biol; Inhibition of STAT3-ferroptosis negative regulatory axis suppresses tumor growth and alleviates chemoresistance in gastric cancer.; Authors - Shumin Ouyang
human	WB	Sci Total Environ; TFEB coordinates autophagy and pyroptosis as hepatotoxicity responses to ZnO nanoparticles; Authors - Xingyao Pei
frog	WB	Proc Natl Acad Sci U S A; Host-derived, pore-forming toxin-like protein and trefoil factor complex protects the host against microbial infection.; Authors - Yang Xiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Weihao (Verified Customer) (02-03-2022)

Works well for mouse BMDM and human macrophages, recommend it

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500

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