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CoraLite®594-conjugated CTP synthase Polyclonal antibody

CTP synthase Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-15914

Synonyms

CTPS, UTP--ammonia ligase 1, EC:6.3.4.2, CTPS1, CTPS 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated CTP synthase Polyclonal an (CL594-15914)

    IF Staining of HepG2 using CL594-15914

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 CTP synthase antibody (CL594-15914) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated CTP synthase Polyclonal an (CL594-15914)

    FC experiment of HepG2 using CL594-15914

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CTP synthase (CL594-15914) (red), or 0.4 ug CoraLite®594-conjugated Rabbit IgG control Rabbit PolyAb (CL594-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-15914 targets CTP synthase in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CTP synthase fusion protein Ag8707
Full Name CTP synthase
Calculated Molecular Weight 591 aa, 67 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC009408
Gene Symbol CTPS
Gene ID (NCBI) 1503
RRIDAB_2919826
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

CTP synthase(CTPS) is also named as CTPS1 and belongs to the CTP synthase family. It catalyses the ATP-dependent formation of CTP from UTP using either L-glutamine or NH3 as the nitrogen source(PMID:12752439). It is important in the biosynthesis of phospholipids and nucleic acids, and plays a key role in cell growth, development, and tumorigenesis (PMID:8813694). CTP synthetase exists as a dimer in the absence of its substrates ATP and UTP, but in the presence of saturating concentrations of these substrates the enzyme exists as a tetramer.(PMID:18439916)

Protocols

Product Specific Protocols
IF protocol for CL594 CTP synthase antibody CL594-15914Download protocol
Standard Protocols
Click here to view our Standard Protocols