Alpha E-Catenin Monoclonal antibody

Alpha E-Catenin Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

2B7B8

Cat no : 66221-1-Ig

Synonyms

CTNNA1, Catenin alpha 1, CAP102, Alpha E catenin, alpha catenin

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 66221-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66221-1-Ig (Alpha E catenin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 66221-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66221-1-Ig (Alpha E catenin antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach using 66221-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66221-1-Ig (Alpha E catenin antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon using 66221-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66221-1-Ig (Alpha E catenin antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 66221-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66221-1-Ig (Alpha E catenin antibody at dilution of 1:200 (under 40x lens).

• 

  FC experiment of MCF-7 using 66221-1-Ig

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human Alpha E-Catenin (66221-1-Ig, Clone:2B7B8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells
• Positive IHC detected in: human stomach tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

66221-1-Ig targets Alpha E-Catenin in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Alpha E-Catenin fusion protein Ag23603
• Full Name: catenin (cadherin-associated protein), alpha 1, 102kDa
• Calculated Molecular Weight: 906 aa, 100 kDa
• Observed Molecular Weight: 95-100 kDa
• GenBank Accession Number: BC031262
• Gene Symbol: Alpha E-Catenin
• Gene ID (NCBI): 1495
• RRID: AB_2881612
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alpha catenin is an essential component of adherens junctions that connects E-cadherin-β-catenin complexes with the actin cytoskeleton. It also recruits a range of other important proteins to developing intercellular junctions. Three alpha catenins exist in human: alpha-E-catenin, alpha-N-catenin, and alpha-T-catenin, which share substantial amino-acid sequence similarity but have distinct tissue distribution. alpha-E-catenin is ubiquitously expressed, alpha-N-catenin is restricted to neuronal tissue, and alpha-T-catenin is primarily expressed in heart tissue. Reduced levels of alpha-E-catenin protein seem to be characteristic of many different human cancers, including malignant tumours of the breast, colon, stomach, oesophagus, bladder and liver. In addition, the loss of alpha-E-catenin often correlates with the degree of tumour differentiation and metastasis.

Publications

Species	Application	Title
mouse	IHC	Int J Mol Sci; Histological and Immunohistochemical Studies to Determine the Mechanism of Cleft Palate Induction after Palatal Fusion in Mice Exposed to TCDD.; Authors - Chisato Sakuma
human	WB	Oncotarget; YAP1 enhances cell proliferation, migration, and invasion of gastric cancer in vitro and in vivo.; Authors - Dan Sun
human	WB,IHC	Mol Med Rep; Wild-type KRAS inhibits the migration and invasion of pancreatic cancer through the Wnt/β-catenin pathway; Authors - Xianhua Hu