CTHRC1 Recombinant antibody

CTHRC1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240442E1

Cat no : 83629-1-RR

Synonyms

UNQ762/PRO1550, Protein NMTC1, Collagen triple helix repeat-containing protein 1, collagen triple helix repeat containing 1, 240442E1

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Validation Data Gallery

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  WB analysis of A375 using 83629-1-RR

  A375 cells were subjected to SDS PAGE followed by western blot with 83629-1-RR (CTHRC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83629-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83629-1-RR against Human CTHRC1 were performed. The affinity constant is 72.9 pM.

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Tested Applications

• Positive WB detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83629-1-RR is CTHRC1 Fusion Protein expressed in E. coli.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CTHRC1 fusion protein Ag9812
• Full Name: collagen triple helix repeat containing 1
• Calculated Molecular Weight: 243 aa, 26 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC014245
• Gene Symbol: CTHRC1
• Gene ID (NCBI): 115908
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Collagen triple helix repeat-containing protein 1 (CTHRC1), also known as protein NMTC1, is a glycosylated secreted protein. CTHRC1 was identified as a novel molecular in injured and diseased arteries where it may contribute to vascular remodeling by limiting collagen matrix deposition and promoting cell migration (PMID: 15618538). It has been reported that CTHRC1 is a positive regulator of osteoblastic bone formation (PMID: 18779865). Besides, studies reveal that CTHRC1 expression is implicated in various malignancies, including hepatocellular carcinoma, gastric carcinoma, colon cancer, and lung cancer (PMID: 23922981; 24504172; 24746208; 25139095).