CTHRC1 Recombinant antibody

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  WB analysis of A375 using 83629-1-RR

  A375 cells were subjected to SDS PAGE followed by western blot with 83629-1-RR (CTHRC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of A375 using 83629-1-RR

  1x10^6 A375 cells were intracellularly stained with 0.25 ug CTHRC1 Recombinant antibody (83629-1-RR, Clone:240442E1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83629-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83629-1-RR against Human CTHRC1 were performed. The affinity constant is 72.9 pM.

CTHRC1 {ptg:Brand} Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 83629-1-RR

Clone No.240442E1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

UNQ762/PRO1550, Protein NMTC1, Collagen triple helix repeat-containing protein 1, collagen triple helix repeat containing 1, 240442E1

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Tested Applications

• Positive WB detected in: A375 cells
• Positive FC (Intra) detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83629-1-RR targets CTHRC1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CTHRC1 fusion protein Ag9812
• Full Name: collagen triple helix repeat containing 1
• Calculated Molecular Weight: 243 aa, 26 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC014245
• Gene Symbol: CTHRC1
• Gene ID (NCBI): 115908
• RRID: AB_3671242
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q96CG8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Collagen triple helix repeat-containing protein 1 (CTHRC1), also known as protein NMTC1, is a glycosylated secreted protein. CTHRC1 was identified as a novel molecular in injured and diseased arteries where it may contribute to vascular remodeling by limiting collagen matrix deposition and promoting cell migration (PMID: 15618538). It has been reported that CTHRC1 is a positive regulator of osteoblastic bone formation (PMID: 18779865). Besides, studies reveal that CTHRC1 expression is implicated in various malignancies, including hepatocellular carcinoma, gastric carcinoma, colon cancer, and lung cancer (PMID: 23922981; 24504172; 24746208; 25139095).