CTBP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CTBP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

9

Reviews (1)

Cat no : 10972-1-AP

Synonyms

EC:1.1.1.-, C-terminal-binding protein 1, CTBP, BARS

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Validation Data Gallery

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  WB analysis using 10972-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10972-1-AP (CTBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10972-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10972-1-AP (CTBP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 10972-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10972-1-AP (CTBP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10972-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10972-1-AP (CTBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 10972-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10972-1-AP (CTBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10972-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10972-1-AP (CTBP1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10972-1-AP

  IP result of anti-CTBP1 (IP:10972-1-AP, 3ug; Detection:10972-1-AP 1:200) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human prostate cancer using 10972-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10972-1-AP (CTBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10972-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10972-1-AP (CTBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10972-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CTBP1 antibody (10972-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 10972-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CTBP1 antibody (10972-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HepG2 using 10972-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human CTBP1 (10972-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse skeletal muscle tissue, mouse thymus tissue, mouse brain tissue, HepG2 cells, Jurkat cells, NIH/3T3 cells, SH-SY5Y cells, HeLa cekks
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IF: See 2 publications below

Product Information

10972-1-AP targets CTBP1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CTBP1 fusion protein Ag1425
• Full Name: C-terminal binding protein 1
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC011655
• Gene Symbol: CTBP1
• Gene ID (NCBI): 1487
• RRID: AB_2086628
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTBP1, also named as C-terminal-binding protein 1, is a 440 amino acid protein, which belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. CTBP1 is a cellular phosphoprotein that associates with various proteins and functions as a corepressor of transcription. CTBP1 and the related protein CTBP2 are characterized as C-terminal binding protein of adenovirus E1A, and they preferentially associate with the E1A via a 5-amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CTBP1 is expressed from embryo to adult, but CTBP2 is mainly expressed during embryogenesis.

Publications

Species	Application	Title
mouse	WB	Nat Commun; CDYL suppresses epileptogenesis in mice through repression of axonal Nav1.6 sodium channel expression.; Authors - Yongqing Liu
human	WB	Nat Commun; ZNF516 suppresses EGFR by targeting the CtBP/LSD1/CoREST complex to chromatin.; Authors - Lifang Li ; KD Validated
human	WB,IF	Oncogene; RBBP8/CtIP suppresses P21 expression by interacting with CtBP and BRCA1 in gastric cancer.; Authors - Yang Yu
human	WB	Mol Oncol; Role of hepatoma-derived growth factor in promoting de novo lipogenesis and tumorigenesis in hepatocellular carcinoma.; Authors - Xuejie Min
human	WB	Mol Carcinog; Role of miR-214 in regulation of β-catenin and the malignant phenotype of melanoma.; Authors - Kirthana Prabhakar ; KD Validated
mouse	WB	Front Physiol; Ling-gui-zhu-gan granules reduces obesity and ameliorates metabolic disorders by inducing white adipose tissue browning in obese mice; Authors - Yuxiu Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tongbin (Verified Customer) (02-18-2020)

This antibody worked perfectly. I had a specific band at ~48kD, which is at the predicted size of Ctbp1. Highly recommended for detecting Ctbp1 by western blots!

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart

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