CSNK2B Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CSNK2B Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B5A6

Cat no : 67866-1-PBS

Synonyms

Casein Kinase 2 beta, Casein kinase II subunit beta, CK II beta, CK2B, CK2N, CSK2B, CSNK2B, G5A, Phosvitin, Protein G5a

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Validation Data Gallery

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  WB analysis using 67866-1-Ig (same clone as 67866-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67866-1-Ig (CSNK2B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 67866-1-Ig (same clone as 67866-1-PBS)

  WB result of CSNK2B antibody (67866-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CSNK2B transfected MCF-7 cells. This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67866-1-Ig (same clone as 67866-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67866-1-Ig (CSNK2B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67866-1-Ig (same clone as 67866-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67866-1-Ig (CSNK2B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67866-1-Ig (same clone as 67866-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67866-1-Ig (CSNK2B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67866-1-Ig (same clone as 67866-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67866-1-Ig (CSNK2B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67866-1-Ig (same clone as 67866-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CSNK2B antibody (67866-1-Ig, Clone: 1B5A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67866-1-PBS targets CSNK2B in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CSNK2B fusion protein Ag19180
• Full Name: casein kinase 2, beta polypeptide
• Calculated Molecular Weight: 215 aa, 25 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC112017
• Gene Symbol: CSNK2B
• Gene ID (NCBI): 1460
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CSNK2B is a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. It participates in Wnt signaling, and plays a complex role in regulating the basal catalytic activity of the alpha subunit.