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  • Featured Product
  • KD/KO Validated

CSNK2B Monoclonal antibody

CSNK2B Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B5A6

Cat no : 67866-1-Ig

Synonyms

Casein Kinase 2 beta, Casein kinase II subunit beta, CK II beta, CK2B, CK2N, CSK2B, CSNK2B, G5A, Phosvitin, Protein G5a

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CSNK2B Monoclonal antibody (67866-1-Ig)

    WB analysis using 67866-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67866-1-Ig (CSNK2B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using CSNK2B Monoclonal antibody (67866-1-Ig)

    WB analysis of MCF-7 using 67866-1-Ig

    WB result of CSNK2B antibody (67866-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CSNK2B transfected MCF-7 cells.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CSNK2B Monoclonal antibody (67866-1-Ig)

    IHC staining of human breast cancer using 67866-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67866-1-Ig (CSNK2B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CSNK2B Monoclonal antibody (67866-1-Ig)

    IHC staining of human breast cancer using 67866-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67866-1-Ig (CSNK2B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CSNK2B Monoclonal antibody (67866-1-Ig)

    IHC staining of human colon cancer using 67866-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67866-1-Ig (CSNK2B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CSNK2B Monoclonal antibody (67866-1-Ig)

    IHC staining of human colon cancer using 67866-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67866-1-Ig (CSNK2B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CSNK2B Monoclonal antibody (67866-1-Ig)

    IF Staining of HeLa using 67866-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CSNK2B antibody (67866-1-Ig, Clone: 1B5A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inA549 cells, MCF-7 cells, LNCaP cells, HeLa cells, Jurkat cells, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67866-1-Ig targets CSNK2B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CSNK2B fusion protein Ag19180
Full Name casein kinase 2, beta polypeptide
Calculated Molecular Weight 215 aa, 25 kDa
Observed Molecular Weight27 kDa
GenBank Accession NumberBC112017
Gene Symbol CSNK2B
Gene ID (NCBI) 1460
RRIDAB_2918624
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSNK2B is a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. It participates in Wnt signaling, and plays a complex role in regulating the basal catalytic activity of the alpha subunit.

Protocols

Product Specific Protocols
WB protocol for CSNK2B antibody 67866-1-IgDownload protocol
IHC protocol for CSNK2B antibody 67866-1-IgDownload protocol
IF protocol for CSNK2B antibody 67866-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols