CSF2RA/CD116 Recombinant antibody, PBS Only (Detector)

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  WB analysis using 84023-1-RR (same clone as 84023-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84023-1-RR (CSF2RA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84023-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00953-1

  Sandwich ELISA standard curve of MP00953-1, Human CSF2RA/CD116 Recombinant Matched Antibody Pair - PBS only. 98066-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02335. 84023-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP00953-1

  The mean CSF2RA/CD116 concentration was determined to be 84.1 ng/mL in THP-1 cell extract based on a 4.0 mg/mL extract load and 59.0 ng/mL in HL-60 cell extract based on a 5.6 mg/mL extract load.

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  Sample test of MP00953-1

  Serum of six individual healthy human donors was measured. The CSF2RA/CD116 concentration of detected samples was determined to be 2.0 ng/mL with a range of 0.9-4.7 ng/mL.

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  IHC staining of human placenta using 84023-1-RR (same clone as 84023-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84023-1-RR (CSF2RA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84023-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84023-1-RR (same clone as 84023-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84023-1-RR (CSF2RA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84023-1-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 84023-1-RR (same clone as 84023-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CSF2RA antibody (84023-1-RR, Clone: 241266A9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84023-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00953-1

  Cytometric bead array standard curve of MP00953-1, CSF2RA/CD116 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 98066-1-PBS. Detection antibody: 84023-1-PBS. Standard: RP02335. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84023-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84023-1-RR against Human CSF2RA/CD116 were performed. The affinity constant is 1.05 nM.

CSF2RA/CD116 {ptg:Brand} Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84023-1-PBS

Clone No.241266A9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CD116, 241266A9, GMCSFR, GM CSF R alpha, CSF2RA

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Product Information

84023-1-PBS targets CSF2RA/CD116 as part of a matched antibody pair:

MP00953-1: 98066-1-PBS capture and 84023-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage)
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC002635
• Gene Symbol: CD116
• Gene ID (NCBI): 1438
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P15509
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

CD116, also named as CSF2RA CDw116 CSF2RAX CSF2RAY CSF2RX CSF2RY GM-CSF-R-alpha GMCSFR and GMR, is a low affinity receptor for granulocyte-macrophage colony-stimulating factor. CD116 transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells.