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  • Western Blot (WB) analysis of various lysates using CSF2RA/CD116 Recombinant antibody (84023-1-RR)

    WB analysis using 84023-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84023-1-RR (CSF2RA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CSF2RA/CD116 Recombinant antibody (84023-1-RR)

    IHC staining of human placenta using 84023-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84023-1-RR (CSF2RA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CSF2RA/CD116 Recombinant antibody (84023-1-RR)

    IHC staining of human placenta using 84023-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84023-1-RR (CSF2RA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using CSF2RA/CD116 Recombinant antibody (84023-1-RR)

    IF Staining of THP-1 using 84023-1-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CSF2RA antibody (84023-1-RR, Clone: 241266A9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84023-1-RR

    Affinity and Kinetic Characterization of 84023-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84023-1-RR against Human CSF2RA/CD116 were performed. The affinity constant is 1.05 nM.

CSF2RA/CD116 Recombinant antibody

CSF2RA/CD116 {ptg:Brand} Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84023-1-RR
Clone No.241266A9

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CD116, 241266A9, GMCSFR, GM CSF R alpha, CSF2RA

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inTHP-1 cells, HL-60 cells, U-937 cells, human placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84023-1-RR targets CSF2RA/CD116 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein Predict reactive species
Full Name colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage)
Calculated Molecular Weight 46 kDa
Observed Molecular Weight60-70 kDa
GenBank Accession NumberBC002635
Gene Symbol CD116
Gene ID (NCBI) 1438
RRIDAB_3671592
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP15509
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD116, also named as CSF2RA CDw116 CSF2RAX CSF2RAY CSF2RX CSF2RY GM-CSF-R-alpha GMCSFR and GMR, is a low affinity receptor for granulocyte-macrophage colony-stimulating factor. CD116 transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells.

Protocols

Product Specific Protocols
WB protocol for CSF2RA/CD116 antibody 84023-1-RRDownload protocol
IHC protocol for CSF2RA/CD116 antibody 84023-1-RRDownload protocol
IF protocol for CSF2RA/CD116 antibody 84023-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols