Anti-Human CRTAM/CD355 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using 98157-1-RR

  1x10^6 untreated or PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CRTAM/CD355 Rabbit Recombinant Antibody (98157-1-RR, Clone: 241280B12) and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with CoraLite® Plus 647 Anti-Human CD8. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98157-1-RR

  1x10^6 PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CRTAM/CD355 Rabbit Recombinant Antibody (98157-1-RR, Clone: 241280B12) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CRTAM/CD355 Rabbit Recombinant Antibody (98157-1-RR, Clone: 241280B12) and PE-conjugated Goat Anti-Rabbit IgG (dashed, black). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98157-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98157-1-RR against Human CRTAM/CD355 were performed. The affinity constant is 0.449 nM.

CRTAM/CD355 {ptg:Brand} Recombinant Antibody for FC

Cat No. 98157-1-RR

Clone No.241280B12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CRTAM, Cytotoxic and regulatory T-cell molecule, Class-I MHC-restricted T-cell-associated molecule, CD355, 241280B12

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Tested Applications

• Positive FC detected in: PMA and ionomycin treated human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98157-1-RR targets CRTAM/CD355 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: cytotoxic and regulatory T cell molecule
• Calculated Molecular Weight: 45 kDa
• GenBank Accession Number: NM_019604.4
• Gene Symbol: CRTAM
• Gene ID (NCBI): 56253
• RRID: AB_3672300
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O95727-1
• Storage Buffer: PBS with 0.09% sodium azide , pH 7.3
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Cytotoxic and regulatory T cell molecule (CRTAM, also known as CD355) is an immunoglobulin-superfamily transmembrane protein with V and C1-like Ig domains, which is involved in epithelial cell adhesion (PMID: 18329370; 20556794). CRTAM is critical to instruct the differentiation of CD4+ cytotoxic T cells (CTL) through the induction of eomesodermin and CTL-related genes (PMID: 26694968). It also regulates CD8+ T cell retention within the lymph node and eventually effector function (PMID: 19752223).