CRTAC1 Monoclonal antibody, PBS Only

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  WB analysis using 68276-1-Ig (same clone as 68276-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68276-1-Ig (CRTAC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68276-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68276-1-Ig (same clone as 68276-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CRTAC1 antibody (68276-1-Ig, Clone: 2A11E2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68276-1-PBS in a different storage buffer formulation.

CRTAC1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 68276-1-PBS

Clone No.2A11E2

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse, rabbit, pig

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ASPIC, ASPIC1, cartilage acidic protein 1, CEP 68, CEP68, CRTAC1, FLJ10320

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Product Information

68276-1-PBS targets CRTAC1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat, mouse, rabbit, pig samples.

• Tested Reactivity: Human, rat, mouse, rabbit, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CRTAC1 fusion protein Ag32813
• Full Name: cartilage acidic protein 1
• Calculated Molecular Weight: 661 aa, 71 kDa
• Observed Molecular Weight: 71 kDa
• GenBank Accession Number: BC034245
• Gene Symbol: CRTAC1
• Gene ID (NCBI): 55118
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NQ79
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CRTAC1 (cartilage acidic protein 1) is also named as ASPIC1 and CEP68. CRTAC1 is an extracellular matrix protein of human chondrogenic tissue. CRTAC1 promotes cell proliferation, migration and extracellular matrix regeneration and remodeling in primary human fibroblasts (PMID:32084494). CRTAC1 expression is decreased in tissue samples of bladder urothelial carcinoma (PMID:34818994). CRTAC1 overexpression promotes the pyroptosis of human lens epithelial cells by facilitating the reactive oxygen species (ROS) production in the formation of cataract (PMID:32838966). CRTAC1 was the most compelling and robust biomarker for osteoarthritis severity and progression (PMID:35924962).